The updated list of authors and affiliations is:Ĭhyi-Ying A. Chang to the author list in the penultimate position. (RMSD) between the experimental and fitted values. We also quantified the goodness of fit by evaluating the root-mean-square deviation Yielding better estimates of the half-lives. The C term in the equation we used provided more freedom for variation in the first time point, thus This equation was modified from a first-order exponential decay model described in Imamachi et al. We characterized each transcript's decay characteristics by nonlinear least-squares fitting of the normalized values to theĮquation: Y(t) = X(t)/X(0) = C*exp(−at), where C (initial intensity) and a (decay rate constant) are floating parameters.
Briefly, for estimating decay rates from the normalized values from time-course and RNA-seq experiments, We now include the script and instruction for calculation of mRNA half-life (see below: § The R Script for calculating mRNA It should be noted as transcripts per million.ħ, under RNA-sequencing and decay rate calculation: The statement “Transcripts whose half-life estimates could notīe accurately determined due to a high residual value…in linear regression analysis were further filtered out” should be changedĪs follows: “We estimated the transcript half-life (t 1/2) by fitting the normalized values to the equation Y(t) = X(t)/X(0) = C*exp(−at) using a nonlinear least-squares estimation Genome Res 19:1639–1645.Ħ, under RNA-sequencing and decay rate calculation: TPM is described as transcripts per kilobase million but, instead, Circos: an information aesthetic for comparative genomics.
REDDIT HALF LIFE SOFTWARE
The changes are detailed as follows:Ģ, line 4 from the bottom (left): “…using circular plots generated with CIRCOS software (Hu et al. These changes do not affect the interpretations of the dataĪnd conclusions of the study. Previously credited in the paper's “Acknowledgments” section). Chang, who performed the related bioinformatics analysisįor panels C and D of Figure 7 and is providing these corrections with additional information, as a coauthor (Dr. I have no desire to go back and finish it, or play Episode I or II.We are submitting this corrigendum to correct a few minor errors and add more detail about the bioinformatics method for mRNA After this, I exited the game and haven't played it since. About 4 months ago I got killed somewhere near the end of the game. Worst $50 I've ever spent, since the only good part of the whole thing is Portal. I bought the Orange Box back in January after hearing how great it was. It's impossible to aim with them because instead of your spray pattern being affected by how long you hold the trigger, it follows some kind of pattern. With any automatic weapon, it seems that at best 1/3 shots hit their mark. The only thing that would get me to consider playing the sequels would be if I got to go on a murderous rampage killing all of my allies. He'll do all of our work for us because we're lazy/incompetent/useless." If I was Freeman, I'd just let everyone die. Seriously, every five minutes it's "Oh, look, Freeman's here. If it were possible, I would have killed all of my allies within the first 5 seconds of meeting any one of them. Then on top of that you have annoying characters. But the vehicle part of the game was only the first thing that made me hate it. Those parts are long, boring, and frustrating due to the horrible controls. First of all, the parts where you have to ride around in vehicles are horrible.
If you're a sci-fi FPS fan who likes thought-provoking video games, I wouldn't be surprised to hear after playing HL2 you'll want to come back and give this one another shot =). If you can't get over how bad the graphics are, you might try Half-Life 2 first instead. The graphics are obviously bad, considering the games age, but if you can look past that, this game is no doubt a gem. If you like science-fiction and FPS games, this is definitely worth considering. Certain parts of the game were definitely frustrating, but shouldn't stop you from enjoying the game if you have the drive to finish it. The atmosphere is incredible, and the combat system is fun. I didn't get into it as much at first until I played Half-Life 2, but after I came back to it, even after all these years, I found that the world was just unforgettably good. I didn't get into it as much at first until I played Half-Life 2, but after I came back to it, even after all these years, I Classic FPS.